MQ07KER Rapid tests for the determination of residual amounts of quinolones in meat, offal and seafood

The kit is used to determine quinolones in animal MEAT, including poultry, offal andseafood (fish, shrimp, shellfish,oysters ).

Storage conditions:

Test kits should be stored at 2-8 °C.  Do not freeze!

Shelf life: 18 months. The batch number and expiration date are indicated on the packaging.

Operating principle:

The reagent kit uses an immunochromatographic method using colloidal gold particles. The analysis consists of two stages: sample preparation and detection of quinolones. The prepared sample is added to the well with antibodies, if quinolones are present in the sample, they will bind to the antibodies, thus preventing subsequent binding of antibodies to antigens applied to the nitrocellulose membrane of the test strip. The result of the reaction is the coloring of the strips, which is taken into account later.

Detection limit of the test kit:

Set composition:

6 tubes, each containing 1 strip with 8 wells with a reagent containing antibodies and 8 test strips (48 tests in total);

1 a bottle of  concentrated Buffer A for sample dilution (5x concentrate);

1 bottle of Buffer B for sample dilution;

Instructions;

Materials required:

Reading device (if necessary);

Scales , vortex, homogenizer , centrifuge , 15 ml or 50 ml centrifuge tubes, dispenser (100-1000 µl).

Preparation of working solution of Buffer A for sample dilution

Mix concentrated Buffer A to dilute the sample with distilled water in a ratio of 1:4. Mix well.

Sample preparation:

Samples for analysis are taken in accordance with STB 1036, GOST 34668 or current TNPA. The samples taken can be stored in a place protected from light at a temperature of plus 2 ° C to plus 4 ° C for 2 days or frozen at a temperature not exceeding minus 20 ° C for 14 days. Before sample preparation, frozen samples must be defrosted at a temperature of plus 2 ° C to plus 4 ° C.

Bring the temperature of the samples from plus 20 °C to plus 25 °C, keeping them at room temperature, then homogenize them using a homogenizer. The samples under study must be uniform.

From the homogenized sample, take a sample weighing  2.0 + 0.1 g . Place the sample in a 15 ml or 50 ml centrifuge tube and add  2 ml  of the working solution of Buffer A to dilute the sample (keep shrimp samples in a water bath at 85℃ for 5 minutes). Vortex the contents of the tube for 2 minutes. And then centrifuge the tube with the sample in the following mode: 4000 rpm for 3-5 minutes.

Take  40 µl  of the supernatant into a test tube, add  700 µl  of Buffer B to dilute the sample, mix well. The prepared solution is used for subsequent analysis and is a test sample.

Analysis procedure:

All components of the kit should be at room temperature (20-25 °C). Prepare the required number of wells with reagent and test strips. Unused reagents and components of the kit should be put in the refrigerator.

Place the well with the reagent in the well plate. Add 100 µl  of the test sample to the well with the reagent  . Mix the sample with the reagent thoroughly by five to six times filling and draining the liquid using the dispenser in the well until uniform pink coloring and incubate the mixture for  2 minutes .

Then place the test strip from the kit into the well with the reagent and sample and incubate for another 5 minutes.

After incubation, remove the test strip from the well with the reagent and sample, remove the porous sponge and interpret the result within 3 minutes.