The reference laboratory for viral diseases of birds of the Federal State Budgetary Institution "ARRIAH" took part in international comparative tests to determine antibodies to infectious bursal disease virus

The Reference Laboratory of Viral Diseases of Birds of the Federal State Budgetary Institution "Federal Center for Animal Health" subordinated to the ROSSELKHOZNADZOR took part in international comparison tests (ICTs) organized by the laboratory of the Dutch Animal HEALTH Service (R&D GD), Deventer, the Netherlands, which is officially accredited in in accordance with the international requirements iso 17043:2010 for the organization of ISI for a number of infectious animal diseases.

68 laboratories from 32 countries participated in the ISI round to determine the status of chicken blood serum samples for the presence of antibodies to infectious bursal disease virus.

A panel was obtained, consisting of eight encrypted pooled samples of chicken blood sera: from 16-week-old SPF laying hens vaccinated twice with the Bursine vaccine, samples were taken 14 days after vaccination; 39-day SPF laying hens vaccinated with Nobilis REO+IB+IBD+ND, 43 days after vaccination; 12 week SPF in broiler parent stock vaccinated with Hipra Gum GM/97 10 days post-vaccination; from chickens vaccinated in ovo with MB1 and infected on day 36 with vvIBDV D6948, samples were taken on day 46 after infection; 4-week-old SPF broilers infected with avirulent virus serotype 2 (WTY89-3/251113) 34 days after infection; 50-week-of-age SPF of broiler parent stock; 12-week-old SPF broilers vaccinated with Hipra Gum CH/80 10 days after vaccination and SPF broilers, infected with the European genetic variant of the IBD virus, 11 days after infection, samples were taken on the 46th day after infection. Laboratory staff tested encrypted samples using commercial kits manufactured by FGBI "ARRIAH" for the detection of antibodies to infectious bursal disease virus by enzyme immunoassay when testing samples in one dilution.

In all cases, the samples were identified correctly: in five samples from vaccinated birds and one sample from chickens infected with a genetic variant of the IBD virus, antibodies to the infectious bursal disease virus were found. Data on the results of the MSI are reflected in the summary report provided by the Dutch Animal Health Service, Deventer (R&D GD), laboratory proficiency testing provider. Received a certificate of the established sample.

Comparative evaluation of the results showed that of all participants, 5 laboratories (7%) encountered difficulties in determining the status of the samples: one laboratory received a false negative result in the determination of a serum sample from chickens vaccinated with Hipra GM/97 vaccine, two laboratories received a false negative result in the determination of the sample sera from broilers vaccinated with live Hipra Gum CH/80 vaccine, one laboratory received a false-negative result in a serum sample from chickens vaccinated with Bursine 2 vaccine, one laboratory received a false-negative result in a serum sample from chickens infected with a field genetic variant of the virus.

Press service of the FGBI "ARRIAH"